PKM2 Protein (His tag)

Catalog No. ABIN7319403

Product Details

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PKM2 protein is labelled with His tag.

Purpose: Recombinant Human PKM2 Protein (His Tag)

Sequence: Ser2-Pro531

Characteristics: Recombinant Human Pyruvate kinase isoform M2 is produced by our E.coli expression system and the target gene encoding Ser2-Pro531 is expressed with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of PBS, pH 7.0, 10 % glycerol.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2 (PKM2 Products)

Synonyms: CTHBP Protein, OIP3 Protein, PK3 Protein, PKM2 Protein, TCB Protein, THBP1 Protein, AA414905 Protein, AL024370 Protein, AL024424 Protein, Pk-2 Protein, Pk-3 Protein, Pk3 Protein, Pkm2 Protein, PKM12 Protein, PKM Protein, pyruvate kinase M1/2 Protein, pyruvate kinase, muscle Protein, PKM Protein, Pkm Protein

Background:

Background: Pyruvate kinase isozymes M2 also known as pyruvate kinase muscle isozyme 2 (PKM2), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an isoenzyme of the glycolytic enzyme pyruvate kinase. Pyruvate kinase isozymes M2 / PKM2 is a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. PKM2 has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. PKM2 functions as a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. PKM2 may stimulates POU5F1-mediated transcriptional activation. This protein Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms of PKM2 contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Synonym: CTHBP,HEL-S-30,OIP3,PK3,PKM2,TCB,THBP1

Molecular Weight: 60.1 kDa

Pathways: Warburg Effect