ERBB3 Protein (AA 730-1065) (His-GST)

Catalog No. ABIN7197657

This Recombinant ERBB3 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for ERBB3 Protein (AA 730-1065) (His-GST) (ABIN7197657)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Bio-Layer Interferometry (BLI)

Purity: > 75 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 730-1065

Purification tag / Conjugate: This ERBB3 protein is labelled with His-GST.

Purpose: Recombinant Human HER3/ErbB3 Protein (aa 730-1065, His &GST Tag)

Sequence: Pro 730-Ser 1065

Characteristics: A DNA sequence encoding the cytoplasmic domain (Pro 730-Ser 1065) of human ErbB3 (NP_001973.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. No Kinase Activity 2. Using the Octet RED System, the affinity constant (Kd) of human ErBB3-GST bound to human NRG1 (aa 2-224) was 20 nM.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.5, 10 % glycerol, 1 mM GSH
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: HER3/ErbB3

Background: EEBB3,ErbB-3,HER3,LCCS2,MDA-BF-1,c-erbB-3,c-erbB3,erbB3-S,p180-ErbB3,p45-sErbB3,p85-sErbB3,ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Molecular Weight:

Calculated MW: 65.0 kDa

Observed MW: 65 kDa

Gene ID: 2065

NCBI Accession: NP_001973

UniProt: P21860

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway