SLPI Protein (AA 26-132) (His tag)

Catalog No. ABIN7197865

Product Details

Target: SLPI (Secretory Leukocyte Peptidase Inhibitor (SLPI))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 26-132

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This SLPI protein is labelled with His tag.

Purpose: Recombinant Human SLPI Protein (aa 26-132, His Tag)(Active)

Sequence: Ser 26-Ala 132

Characteristics: A DNA sequence encoding the secreted form of human SLPI (P03973) (Ser 26-Ala 132) was expressed, with a polyhistidine tag at the N-terminus.

Purity: > 84 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp)-NH2 (Catalog # ES002).The IC50 value is <4 Nm.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SLPI (Secretory Leukocyte Peptidase Inhibitor (SLPI))

Alternative Name: SLPI (SLPI Products)

Synonyms: ALK1 Protein, ALP Protein, BLPI Protein, HUSI Protein, HUSI-I Protein, MPI Protein, WAP4 Protein, WFDC4 Protein, MATN4 Protein, SLPI Protein, ckpi Protein, secretory leukocyte peptidase inhibitor Protein, antileukoproteinase Protein, SLPI Protein, Slpi Protein, LOC102174044 Protein

Background:

Background: Secretory leukoprotease inhibitor (SLPI), also called antileukoprotease (ALP), is a 12- kDa, nonglycosylated serine protease inhibitor present in mucous secretions. It is thought to play a role in protecting the mucosae from injury associated with inflammation. SLPI is locally produced by serous cells, including bronchial submucosal glands. Elafin and SLPI are members of larger families of proteins secreted predominantly at mucosal sites, and have been shown to be modulated in multiple pathological conditions. Elafin and SLPI are structurally related in that both have a fold with a four-disulfide core or whey acidic protein (WAP) domain responsible for inhibiting proteases. SLPI is a prominent innate immune protein of the respiratory tract, possessing serine protease inhibitor activity, antibacterial activity, and anti-inflammatory/immunomodulatory activity.

Synonym: ALK1,ALP,BLPI,HUSI,HUSI-I,MPI,WAP4,WFDC4

Molecular Weight: 14 kDa

UniProt: P03973