SERPINC1 Protein (His tag)

Catalog No. ABIN7197921

Product Details

Target: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SERPINC1 protein is labelled with His tag.

Purpose: Recombinant Human SerpinC1/AntithrombinIII/ATIII Protein (His Tag)(Active)

Sequence: Met 1-Lys 464

Characteristics: A DNA sequence encoding the human SerpinC1 (NP_000479.1) (Met 1-Lys 464) was expressed with a C-terminal polyhistidine tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit thrombin (Sigma, Catalog # T4648)cleavage of a fluorogenic peptide substrate Boc-VPR-AMC.The IC50 value is < 5 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Alternative Name: SerpinC1/AntithrombinIII/ATIII (SERPINC1 Products)

Synonyms: AT3 Protein, AT3D Protein, ATIII Protein, THPH7 Protein, SERPINC1 Protein, DKFZp470C1733 Protein, AI114908 Protein, At-3 Protein, At3 Protein, ANTITHROMBIN, AT-III Protein, AT-III Protein, serpin family C member 1 Protein, antithrombin-III Protein, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 Protein, SERPINC1 Protein, CpipJ_CPIJ000472 Protein, CpipJ_CPIJ013111 Protein, Serpinc1 Protein

Background:

Background: SerpinC1, also known as antithrombin III (AT III), is a member of the serpin superfamily of serine protease inhibitors, and has been found to be a marker for disseminated intravascular coagulation (DIC) and to be of prognostic significance in septic patients. SerpinC1 synthesized in the liver is the principal plasma serpin of blood coagulation proteases and inhibits thrombin and other factors such as Xa by the formation of covalently linked complexes. Thus it is one of the most important coagulation inhibitors and the fundamental enzyme for the therapeutical action of heparin. In common with SerpinA5 and D1, the inhibitory activity of SerpinC1 undergoes a dramatic increase in the presence of heparin and other glycosaminoglycans. ATIII mediates the promotion of prostaglandin release, an inhibitor of leucocyte activation and downregulator of many proinflammatory cytokines. Antithrombin III exerts anti-inflammatory properties in addition to its anti-coagulative mechanisms. In animal models of sepsis, ATIII affected cytokine plasma concentrations with a decrease of pro-inflammatory cytokines. The deficiency or functional abnormality of ATIII may result in an increased risk of thromboembolic disease, such as deep vein thrombosis and pulmonary embolism. In addition, it has been reported that SerpinC1 can alter or influence inflammatory processes via inhibition of NF-κB activation or actin polymerization.

Synonym: AT3;AT3D;ATIII;MGC22579;SerpinC1;THPH7

Molecular Weight: 50.5 kDa

NCBI Accession: NP_000479