STK40 Protein (GST tag,His tag)

Catalog No. ABIN7197936

Product Details

Target: STK40 (serine/threonine Kinase 40 (STK40))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This STK40 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human STK40 Protein (His & GST Tag)

Sequence: Met 1-Lys 435

Characteristics: A DNA sequence encoding the human STK40 (NP_114406) (Met1-Lys435) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 97 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, 3 mM DTT, 0.5M Urea, 0.5 mM GSH, pH 8.0

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: STK40 (serine/threonine Kinase 40 (STK40))

Alternative Name: STK40 (STK40 Products)

Synonyms: cb988 Protein, fd99h11 Protein, wu:fd99h11 Protein, NV14115 Protein, DKFZp459J1924 Protein, RP11-268J15.4 Protein, SHIK Protein, SgK495 Protein, 2310004N11Rik Protein, AI428792 Protein, Lyk4 Protein, RGD1307310 Protein, serine/threonine kinase 40 Protein, serine/threonine kinase 40 L homeolog Protein, STK40 Protein, stk40 Protein, LOC100123745 Protein, Stk40 Protein, stk40.L Protein

Background:

Background: STK40 localized to both the cytoplasm and the nucleus. It is ubiquitously expressed. Mechanistically, Stk40 interacts with Rcn2, which also activates Erk1/2 to induce ExEn specification in mouse ESCs. Stk40 is able to activate the Erk/MAPK pathway and induce extraembryonic-endoderm (ExEn) differentiation in mouse ESCs. Interestingly, cells overexpressing Stk40 exclusively contribute to the ExEn layer of chimeric embryos when injected into host blastocysts. In contrast, deletion of Stk40 in ESCs markedly reduces ExEn differentiation in vitro. STK40 has a central serine/threonine protein kinase domain and is homologous to TRB-3, a protein that regulates activation of MAP kinases and inhibits NFκB-mediated gene transcription. Similarly, overexpression of STK40 inhibits NFκB activation triggered by TNF and also inhibits p53-mediated transcription. There are four named isoforms of STK40 that are produced as a result of alternative splicing.

Synonym: SgK495;SHIK

Molecular Weight: 76.8 kDa

NCBI Accession: NP_114406