SH2D1A Protein (His tag)

Catalog No. ABIN7317640

Product Details

Target: SH2D1A (SH2 Domain Containing 1A (SH2D1A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SH2D1A protein is labelled with His tag.

Purpose: Recombinant Human SH2D1A/SAP Protein (His Tag)(Active)

Sequence: Pro 2-Lys 97

Characteristics: A DNA sequence encoding the human SH2D1A isoform 1 (NP_002342.1) (Pro 2-Lys 97) was expressed, with a polyhistidine tag at the N-terminus.

Purity: > 94 % as determined by reducing SDS-PAGE.

Biological Activity Comment: 1. Measured by its ability to bind recombinant human SLAMF1 in a functional ELISA.2. Measured by its ability to bind recombinant human SLAMF6 in a functional ELISA.3. Measured by its ability to bind recombinant mouse SLAMF6 in a functional ELISA.4. Measured by its ability to bind recombinant mouse CD84 in a functional ELISA.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.5, 20 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SH2D1A (SH2 Domain Containing 1A (SH2D1A))

Alternative Name: SH2D1A/SAP (SH2D1A Products)

Synonyms: DSHP Protein, EBVS Protein, IMD5 Protein, LYP Protein, MTCP1 Protein, SAP Protein, SAP/SH2D1A Protein, XLP Protein, XLPD Protein, RGD1562408 Protein, Gm686 Protein, SH2D1A Protein, SH2 domain containing 1A Protein, SH2D1A Protein, Sh2d1a Protein

Background:

Background: SH2 domain-containing protein 1A (SH2D1A / SAP) is a 128 amino acid protein, containing a single Src homology 2 (SH2) domain, flanked by 5 amino acids at the N-terminus and 25 amino acids at the C-terminus. The absence of a catalytic domain and the presence of an SH2 domain suggest that SH2D1A regulates one or more signal transduction pathways. SH2D1A interacts with signaling lymphocytic activation molecule (SLAM), which is a transmembrane protein expressed on the surface of activated T and B cells. SH2D1A (SAP) interacts via its SH2 domain with a motif (TIYXXV) present in the cytoplasmic tail of the cell-surface receptors, including CD150 / SLAM, CD84, CD229 / Ly-9, and CD244 / 2B4. SH2D1A was expressed in EBV-carrying, tumor phenotype representative (type I), but not in EBV-carrying lymphoblastoid cell line (LCL)-like (type III) or EBV-negative Burkitt lymphoma (BL) lines. It has been supposed to be related to the X-linked lymphoproliferative disease which is also known as Duncan's disease or Purtilo syndrome. 

Synonym: SH2 Domain-Containing Protein 1A; Duncan Disease SH2-Protein; Signaling Lymphocytic Activation Molecule-Associated Protein; SLAM-Associated Protein; T-Cell Signal Transduction Molecule SAP; SH2D1A;DSHP;EBVS;IMD5;LYP;MTCP1;SAP/SH2D1A;XLP;XLPD;XLPD1

Molecular Weight: 15.6 kDa

NCBI Accession: NP_002342

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process