SMAD5 Protein (GST tag,His tag)
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- Target See all SMAD5 Proteins
- SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))
- Protein Type
- Recombinant
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Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This SMAD5 protein is labelled with GST tag,His tag.
- Purpose
- Recombinant Mouse Smad5 Protein (His & GST Tag)
- Sequence
- Thr2-Ser465
- Characteristics
- A DNA sequence encoding the mouse SMAD5 (P97454) (Thr2-Ser465) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Purity
- > 90 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Top Product
- Discover our top product SMAD5 Protein
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SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 186-465) protein (His-IF2DI Tag)
SMAD5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 105-182) protein (GST tag)SMAD5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 1-465) protein (GST tag)SMAD5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 5 (SMAD5) proteinSMAD5 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 2-465) protein (His tag)Crystallography grade SMAD5 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 2-465) protein (His tag)Crystallography grade SMAD5 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))
- Alternative Name
- Smad5 (SMAD5 Products)
- Synonyms
- DWFC Protein, JV5-1 Protein, MADH5 Protein, Madh5 Protein, SBN Protein, fb67b04 Protein, mad Protein, madh5 Protein, sb:cb400 Protein, smd5 Protein, wu:fb67b04 Protein, SMAD5 Protein, DKFZp468M0619 Protein, 1110051M15Rik Protein, AI451355 Protein, Dwf-C Protein, MusMLP Protein, SMAD family member 5 Protein, SMAD5 Protein, Smad5 Protein, smad5 Protein
- Background
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Background: SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
Synonym: 1110051M15Rik,AI451355,Dwf-C,Madh5,MusMLP
- Molecular Weight
- 79.9 kDa
- UniProt
- P97454
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