SRPK1 Protein (GST tag,His tag)

Catalog No. ABIN7317393

Product Details

Target: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This SRPK1 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human SRPK1 Protein (His & GST Tag)

Sequence: Glu 2-Ser 655

Characteristics: A DNA sequence encoding the human SRPK1 (AAH38292.1) (Glu 2-Ser 655) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 80 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Alternative Name: SRPK1 (SRPK1 Products)

Synonyms: SFRSK1 Protein, AU017960 Protein, BEST:CK01209 Protein, CG11489 Protein, CG9085 Protein, CK01209 Protein, Dmel\\CG11489 Protein, SRPK Protein, SRPK1 Protein, Srpk79D Protein, atc Protein, srpk1 Protein, zgc:63789 Protein, wu:fc06h03 Protein, zgc:158800 Protein, sfrsk1 Protein, SRSF protein kinase 1 Protein, serine/arginine-rich protein specific kinase 1 Protein, Serine-arginine protein kinase at 79D Protein, SRSF protein kinase 1a Protein, SRSF protein kinase 1b Protein, SRSF protein kinase 1 S homeolog Protein, SRPK1 Protein, Srpk1 Protein, Srpk79D Protein, srpk1a Protein, srpk1b Protein, srpk1.S Protein

Background:

Background: Serine / threonine-protein kinase SRPK1, also known as SFRS protein kinase 1, Serine/arginine-rich protein-specific kinase 1, SR-protein-specific kinase 1 and SRPK1, is a cytoplasm and nucleus protein which belongs to the protein kinase superfamily and CMGC Ser/Thr protein kinase family. Isoform 2 of SRPK1 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 of SRPK1 is only seen in the testis, at lower levels than isoform 2. SRPK1 hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. SRPK1 plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. SRPK1 locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. SRPK1 appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.

Synonym: RP3-422H11.1;SFRSK1

Molecular Weight: 102 kDa

Pathways: Toll-Like Receptors Cascades