ST6GAL1 Protein (His tag)

Catalog No. ABIN7320160

Product Details

Target: ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This ST6GAL1 protein is labelled with His tag.

Purpose: Recombinant Mouse ST6GAL1 Protein (His Tag)(Active)

Sequence: Lys 27-Cys 403

Characteristics: A DNA sequence encoding the extracellular domain of mouse ST6GAL1 (NP_666045.1) (Lys 27-Cys 403) was fused with a polyhistidine tag at the N-terminus.

Purity: > 96 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to N-Acetyllactosamine.The specific activity is > 150 pmol/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

Alternative Name: ST6GAL1 (ST6GAL1 Products)

Synonyms: SIAT1 Protein, ST6GalI Protein, ST6N Protein, Siat1 Protein, siat1 Protein, zgc:136454 Protein, ST6GAL1 Protein, AW742324 Protein, St6Gal-I Protein, St6gal Protein, St6galI Protein, ST6 beta-galactoside alpha-2,6-sialyltransferase 1 Protein, ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 Protein, beta galactoside alpha 2,6 sialyltransferase 1 Protein, ST6GAL1 Protein, St6gal1 Protein, st6gal1 Protein

Background:

Background: Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein which belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1 / SIAT1 is normally found in the?Golgi?but which can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. β-Galactoside α2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1 / SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.

Synonym: AW742324,Siat1,St6gal,St6Gal-I,St6galI

Molecular Weight: 45.9 kDa

NCBI Accession: NP_666045