SFTPD Protein (Glu22Gly-Mutant) (His tag)

Catalog No. ABIN7318972

Product Details

Target: SFTPD (Surfactant Protein D (SFTPD))

Protein Type: Recombinant

Protein Characteristics: Glu22Gly-Mutant

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This SFTPD protein is labelled with His tag.

Purpose: Recombinant Human SFTPD/SP-D Protein (Glu22Gly, His Tag)

Sequence: Ala21-Phe375(Glu22Gly)

Characteristics: Recombinant Human Pulmonary Surfactant-Associated Protein D is produced by our Mammalian expression system and the target gene encoding Ala21-Phe375(Glu22Gly)? is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SFTPD (Surfactant Protein D (SFTPD))

Alternative Name: SFTPD/SP-D (SFTPD Products)

Synonyms: COLEC7 Protein, PSP-D Protein, SFTP4 Protein, SP-D Protein, BDE Protein, BDSD Protein, HOX4I Protein, SPD Protein, AI573415 Protein, Sftp4 Protein, surfactant protein D Protein, homeobox D13 Protein, surfactant associated protein D Protein, SFTPD Protein, HOXD13 Protein, Sftpd Protein, SP-D Protein

Background:

Background: Surfactant Pulmonary-Associated Protein D (SP-D) is a 43 kDa member of the collectin family of innate immune modulators. Its principal components consist of a collagen-like region and a C-terminal carbohydrate recognition domain (CRD), a structure that places it in a subset of pattern recognition proteins termed defense collagens. SP-D is constitutively secreted by alveolar lining cells and epithelium associated with tubular structures and induced in cardiac smooth muscle and endothelial cells. It binds both secreted and transmembrane proteins that transduce its function. It binds human neutrophil defensins, modulating influenza anti-viral defense. It binds MD-2/LY96, a secreted protein that cooperates with Toll-like receptors (TLRs) in the response of macrophages to bacterial lipopolysaccharides (LPS) or cell wall components. It also binds macrophage CD14 and TLRs directly, blocking binding of LPS and down-regulating TNF-α secretion. SP-D binding of both SIRPα and the calreticulin/CD91 complex on macrophages allows for a graded response to environmental challenge.

Synonym: Pulmonary Surfactant-Associated Protein D, PSP-D, SP-D, Collectin-7, Lung Surfactant Protein D, SFTPD, COLEC7, PSPD, SFTP4,COLEC7,SFTP4

Molecular Weight: 36.5 kDa

UniProt: P35247