SNCA Protein (AA 1-140)

Catalog No. ABIN7320688

Recombinant SNCA protein expressed in Escherichia coli (E. coli).

Quick Overview for SNCA Protein (AA 1-140) (ABIN7320688)

Target: SNCA (Synuclein, alpha (SNCA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-140

Purpose: Recombinant Mouse α-Synuclein/SNCA Protein

Sequence: Met1-Ala140

Characteristics: Recombinant Mouse alpha-Synuclein is produced by our E.coli expression system and the target gene encoding Met1-Ala140 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized Recombinant Mouse SNCA(PKSM041187) at 2μg/ml (100 μl/well) can bind AAnti-Human SNCA antibody.The ED₅₀ of Anti-SNCA antibody is 4.65ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: alpha-Synuclein/SNCA

Background: Alpha-synuclein,NACP,Non-A beta component of AD amyloid,Non-A4 component of amyloid precursor,Snca,Alpha-Synuclein (SNCA) is a member of the Synuclein family. SNCA is expressed principally in brain but also expressed in low concentrations in all tissues except liver. SNCA interacts with UCHL1, Phospholipase D and histones. SNCA can include beta- and gamma-synuclein. In addition, SNCA is an important regulatory component of vesicular transport in neuronal cells. It has been suggested that SNCA is related to the pathogenesis of Parkinson's Disease and neurodegenerative disorders. Defects in SNCA will lead to Dementia Lewy Body (DLB).

Molecular Weight:

Calculated MW: 14.5 kDa

Observed MW: 17 kDa

Gene ID: 20617

UniProt: O55042

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity