TXN Protein (His tag)

Catalog No. ABIN7319079

This Recombinant TXN protein is produced in Escherichia coli (E. coli).

Quick Overview for TXN Protein (His tag) (ABIN7319079)

Target: TXN (Thioredoxin (TXN))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This TXN protein is labelled with His tag.

Purpose: Recombinant Human Thioredoxin/TXN Protein (His Tag)

Sequence: Met 1-Val105

Characteristics: Recombinant Human Thioredoxin is produced by our E.coli expression system and the target gene encoding Met1-Val105 is expressed with a 6His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 1 mM EDTA, 2 mM DTT, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: TXN (Thioredoxin (TXN))

Alternative Name: Thioredoxin/TXN

Background: ADF,ATL-Derived Factor,SASP,Surface-Associated Sulphydryl Protein,TRDX,TRX,TRX1,TXN,Thioredoxin,Trx,Thioredoxin (TXN) is a member of the Thioredoxin family. Thioredoxin exists as a disulfide-linked homodimer and contains one Thioredoxin domain. Thioredoxin is up-regulated by ionizing radiation. Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin also plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide.

Molecular Weight:

Calculated MW: 13.9 kDa

Observed MW: 14 kDa

Gene ID: 7295

UniProt: P10599

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis