TXN2 Protein (His tag)

Catalog No. ABIN7198220

Product Details

Target: TXN2 (Thioredoxin 2 (TXN2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TXN2 protein is labelled with His tag.

Purpose: Recombinant Human Thioredoxin-2/TXN2 Protein (His Tag)(Active)

Sequence: Thr 60-Gly 166

Characteristics: A DNA sequence encoding the mature form of human TXN2 (Q99757) (Thr 60-Gly 166) was expressed, with a polyhistide tag at the N-terminus.

Purity: > 97 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is 5-8 A650/min/mg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 8.3

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TXN2 (Thioredoxin 2 (TXN2))

Alternative Name: Thioredoxin-2/TXN2 (TXN2 Products)

Synonyms: MT-TRX Protein, MTRX Protein, TRX2 Protein, 31884 Protein, CG31884 Protein, CG3864 Protein, DTrx-2 Protein, DmTrx-2 Protein, DmelTrx-2 Protein, Dmel\\CG31884 Protein, Trx Protein, anon-WO0118547.188 Protein, dmtrx-2 Protein, 2510006J11Rik Protein, AI788873 Protein, Trx2 Protein, zgc:77127 Protein, thioredoxin 2 Protein, thioredoxin-2 Protein, thiol reductase thioredoxin Protein, thioredoxin 2 L homeolog Protein, TXN2 Protein, Trx-2 Protein, LOC409451 Protein, VPA0972 Protein, VS_RS18125 Protein, VCD_000568 Protein, VEA_000083 Protein, trx2 Protein, Txn2 Protein, txn2 Protein, txn2.L Protein

Background:

Background: Thioredoxin-2; also known as TXN2; MTRX and TRX2; is a member of the thioredoxin family. Tryparedoxins (TXN) are thioredoxin-related proteins which; as trypanothione:peroxiredoxin oxidoreductases; constitute the trypanothione-dependent antioxidant defense and may also serve as substrates for ribonucleotide reductase in trypanosomatids. Thioredoxin-2 / TXN2 contains one thioredoxin domain. It is widely expressed in adult (at protein level) and fetal tissues. Human Thioredoxin-2 / TXN2 is a small redox protein important in cellular antioxidant defenses; as well as in the regulation of apoptosis. Thioredoxin-2 / TXN2 has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Thioredoxin-2 / TXN2 could be involved in the resistance to anti-tumor agents. It possesses a dithiol-reducing activity. Thioredoxin-2 / TXN2 plays an important role in protecting the mitochondria against oxidative stress and in sensitizing the cells to ROS-induced apoptosis. Mammalian Thioredoxin-2 / TXN2 is a mitochondrial isoform of highly evolutionary conserved thioredoxins. Thioredoxins are small ubiquitous protein-disulfide oxidoreductases implicated in a large variety of biological functions.

Synonym: Thioredoxin Mitochondrial; MTRX; Mt-Trx; Thioredoxin-2; TXN2; TRX2

Molecular Weight: 13.4 kDa

UniProt: Q99757

Pathways: Cell RedoxHomeostasis