TNK2 Protein (GST tag)
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- Target See all TNK2 Proteins
- TNK2 (tyrosine Kinase, Non-Receptor, 2 (TNK2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This TNK2 protein is labelled with GST tag.
- Purpose
- Recombinant Human ACK1/TNK2 Protein (GST Tag)
- Sequence
- Gly 110-Trp 476
- Characteristics
- A DNA sequence encoding the amino acid (Gly 110-Trp 476) of human ACK1 isoform 1 (NP_005772.3) was expressed with the GST tag at the N-terminus.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product TNK2 Protein
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tyrosine Kinase, Non-Receptor, 2 (TNK2) (AA 110-476) (Active) protein (GST tag)
TNK2 Origin: Human Host: Insect cells (Sf21) Recombinant SDS, Func Active
tyrosine Kinase, Non-Receptor, 2 (TNK2) (AA 110-476) (Active) protein (His-GST)TNK2 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
tyrosine Kinase, Non-Receptor, 2 (TNK2) (AA 21-120) protein (GST tag)TNK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
tyrosine Kinase, Non-Receptor, 2 (TNK2) (AA 1-352) protein (GST tag)TNK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
tyrosine Kinase, Non-Receptor, 2 (TNK2) (AA 1-1038) protein (Strep Tag)Crystallography grade TNK2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
tyrosine Kinase, Non-Receptor, 2 (TNK2) (AA 1-1055) protein (Strep Tag)Crystallography grade TNK2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 0.5 mM EDTA, 0.5 mM PMSF, 0.5 mM TCEP
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- TNK2 (tyrosine Kinase, Non-Receptor, 2 (TNK2))
- Alternative Name
- ACK1/TNK2 (TNK2 Products)
- Synonyms
- ACK Protein, ACK-1 Protein, ACK1 Protein, p21cdc42Hs Protein, AW552477 Protein, Ack Protein, Cdgip Protein, Pyk1 Protein, ack1 Protein, ackb Protein, tnk2 Protein, tnk2b Protein, wu:fj16g04 Protein, zgc:154019 Protein, tyrosine kinase non receptor 2 Protein, tyrosine kinase, non-receptor, 2 Protein, tyrosine kinase, non-receptor, 1 Protein, TNK2 Protein, Tnk2 Protein, tnk1 Protein
- Background
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Background: ACK1 (also known as ACK, TNK2, or activated Cdc42 kinase) is a structurally unique non-receptor tyrosine kinase that is expressed in diverse cell types. This downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. The ACK1 protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. ACK1 integrates signals from plethora of ligand-activated receptor tyrosine kinases (RTKs), for example, MERTK, EGFR, HER2 and PDGFR to initiate intracellular signaling cascades. It binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. ACK1 transduces extracellular signals to cytosolic and nuclear effectors such as the protein kinase AKT/PKB and androgen receptor (AR), to promote cell survival and growth. ACK1 participates in tumorigenesis, cell survival, and migration. Gene amplification and overexpression of ACK1 were found in many cancer types such as those of the lung and prostate. Recently, four somatic missense mutations of ACK1, which occur in the N-terminal region, the C-lobe of the kinase domain, and the SH3 domain, were identified in cancer tissue samples.
Synonym: ACK,ACK-1,ACK1,p21cdc42Hs
- Molecular Weight
- 68 kDa
- NCBI Accession
- NP_005772
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