UBA1 Protein (GST tag,His tag)

Catalog No. ABIN7317442

Product Details

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This UBA1 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human UBE1/UBA1 Protein (His & GST Tag)

Sequence: Ser 2-Arg 1058

Characteristics: A DNA sequence encoding the human UBA1 (NP_003325.2) (Ser 2-Arg 1058) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 96 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.4, 10 % glycerol, 0.5 mM GSH

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Alternative Name: UBE1/UBA1 (UBA1 Products)

Synonyms: A1S9 Protein, A1S9T Protein, A1ST Protein, AMCX1 Protein, GXP1 Protein, POC20 Protein, SMAX2 Protein, UBA1A Protein, UBE1 Protein, UBE1X Protein, DDBDRAFT_0190927 Protein, DDBDRAFT_0220497 Protein, DDB_0190927 Protein, DDB_0220497 Protein, Sbx Protein, Ube-1 Protein, Ube1x Protein, a1s9 Protein, a1s9t Protein, a1st Protein, amcx1 Protein, gxp1 Protein, poc20 Protein, smax2 Protein, uba1a Protein, ube1 Protein, ube1x Protein, uba1 Protein, uba1b Protein, ube Protein, zgc:66143 Protein, wu:fa01e08 Protein, wu:fb30f01 Protein, wu:fi21c11 Protein, wu:fj14g11 Protein, ubiquitin like modifier activating enzyme 1 Protein, ubiquitin activating enzyme E1 Protein, Ubiquitin-conjugating enzyme E1 Protein, ubiquitin-like modifier activating enzyme 1 Protein, ubiquitin-like modifier activating enzyme 1 S homeolog Protein, ubiquitin-like modifier activating enzyme 1 L homeolog Protein, UBA1 Protein, uae1 Protein, GL50803_4083 Protein, GL50803_10661 Protein, Uba1 Protein, uba1.S Protein, uba1.L Protein, uba1 Protein, ptr3 Protein

Background:

Background: UBE1, also known as UBA1, belongs to the ubiquitin-activating E1 family. UBE1 gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It also catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Defects in UBA1 can cause spinal muscular atrophy X-linked type 2 (SMAX2), also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.

Synonym: A1S9,A1S9T,A1ST,AMCX1,CFAP124,CTD-2522E6.1,GXP1,POC20,SMAX2,UBA1A,UBE1,UBE1X

Molecular Weight: 146 kDa

NCBI Accession: NP_003325