UBA5 Protein (His tag)
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- Target See all UBA5 Proteins
- UBA5 (Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UBA5 protein is labelled with His tag.
- Purpose
- Recombinant Human UBA5 Protein (His Tag)
- Sequence
- Met 1-Met404
- Characteristics
- Recombinant Human Ubiquitin-fold Modifier 1 Activating Enzyme is produced by our E.coli expression system and the target gene encoding Met1-Met404 is expressed with a 6His tag at the N-terminus.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product UBA5 Protein
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Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5) (AA 197-404) protein (His-IF2DI Tag)
UBA5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5) (AA 1-404) protein (His tag)UBA5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5) (AA 1-404) protein (GST tag)UBA5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5) (AA 1-404) protein (Strep Tag)Crystallography grade UBA5 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBA5 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,50 mM NaCl,1 mM DTT, 10 % glycerol, pH 8.0 .
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- UBA5 (Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5))
- Alternative Name
- UBA5 (UBA5 Products)
- Synonyms
- cb972 Protein, ube1dc1 Protein, UBE1DC1 Protein, THIFP1 Protein, 5730525G14Rik Protein, AW240750 Protein, Ube1dc1 Protein, UBAL Protein, ubiquitin-like modifier activating enzyme 5 Protein, ubiquitin like modifier activating enzyme 5 Protein, ubiquitin-activating enzyme E1-like protein Protein, ubiquitin like modifier activating enzyme 5 S homeolog Protein, uba5 Protein, UBA5 Protein, LOC100270786 Protein, Uba5 Protein, uba5.S Protein
- Background
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Background: UBA5 is a member of the ubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin and ubiquitin-like proteins are recognized as covalently conjugated to various cellular substrates by a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) has a vital role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5 activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. UBA5 is located primarily in cytoplasm, while it generally localizes to the nucleus in presence of SUMO2.
Synonym: Ubiquitin-like modifier-activating enzyme 5, ThiFP1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1, UBE1DC1, UBA5
- Molecular Weight
- 47.0 kDa
- UniProt
- Q9GZZ9
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