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WDYHV1 Protein (GST tag)

WDYHV1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Catalog No. ABIN7318958
  • Target See all WDYHV1 Proteins
    WDYHV1 (WDYHV Motif Containing 1 (WDYHV1))
    Protein Type
    Recombinant
    Origin
    • 3
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 3
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This WDYHV1 protein is labelled with GST tag.
    Purpose
    Recombinant Human WDYHV1/NTAQ1 Protein (GST Tag)
    Sequence
    Met 1-Cys205
    Characteristics
    Recombinant Human Protein N-terminal glutamine amidohydrolase is produced by our E.coli expression system and the target gene encoding Met1-Cys205 is expressed with a GST tag at the N-terminus.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Endotoxin Level
    < 1.0 EU per μg as determined by the LAL method.
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    Discover our top product WDYHV1 Protein
  • Restrictions
    For Research Use only
  • Format
    Frozen, Liquid
    Buffer
    Supplied as a 0.2 μm filtered solution of PBS,100 mM GSH,1 % TritonX-100,15 % Glycerol, pH 7.4.
    Storage
    -20 °C
    Storage Comment
    Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
  • Target
    WDYHV1 (WDYHV Motif Containing 1 (WDYHV1))
    Alternative Name
    WDYHV1/NTAQ1 (WDYHV1 Products)
    Synonyms
    ntaq1 Protein, C8orf32 Protein, 2410187C16Rik Protein, AU014961 Protein, AW550036 Protein, Ntaq1 Protein, RGD1311362 Protein, C14H8orf32 Protein, si:rp71-45k5.3 Protein, WDYHV motif containing 1 Protein, WDYHV motif containing 1 S homeolog Protein, WDYHV1 Protein, wdyhv1.S Protein, wdyhv1 Protein, Wdyhv1 Protein
    Background

    Background: Human protein N-terminal glutamine amidohydrolase (WDYHV1) is an enzyme that in humans is encoded by the WDYHV1 gene, belongs to the NTAQ1 family.WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, which is an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. However,it does not act on substrates with internal or C-terminal glutamine andnon-glutamine residues in any position. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.

    Synonym: Protein N-terminal glutamine amidohydrolase,WDYHV1,Protein NH2-terminal glutamine deamidase,N-terminal Gln amidase,Nt(Q)-amidase,C8orf32, NTAQ1

    Molecular Weight
    49.8 kDa
    UniProt
    Q96HA8
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