WTAP Protein (GST tag)

Catalog No. ABIN7317433

Product Details

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This WTAP protein is labelled with GST tag.

Purpose: Recombinant Human WTAP Protein (GST Tag)

Sequence: Met 1-Arg 151

Characteristics: A DNA sequence encoding the human WTAP isoform 2 (Q15007-2) (Met 1-Arg 151) was fused with the GST tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 0.15M NaCl, 0.5 mM GSH, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Alternative Name: WTAP (WTAP Products)

Synonyms: MGC75954 Protein, WTAP Protein, RGD1563824 Protein, wu:fi29a09 Protein, zgc:66202 Protein, 2810408K05Rik Protein, 9430038B09Rik Protein, WT1 associated protein Protein, Wilms tumor 1 associated protein Protein, wilms tumor 1 associated protein Protein, WT1 associated protein L homeolog Protein, Wilms tumour 1-associating protein Protein, wtap Protein, WTAP Protein, CpipJ_CPIJ006489 Protein, Wtap Protein, wtap.L Protein

Background:

Background: Wilms' tumor 1-associating protein (WTAP) was previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. WT1 was originally identified as a tumor suppressor for Wilms' tumor, but it is also overexpressed in a variety of cancer cells. The WTAP-WT1 axis in vascular cells suggest that WTAP is a vital and multifaceted regulator of vascular remodeling. WTAP has been suggested to function in alternative splicing, stabilization of mRNA, and cell growth. Knocking down endogenous WTAP increased Smooth muscle cells (SMCs) proliferation, because of increased DNA synthesis and G(1)/S phase transition, together with reduced apoptosis. These effects could be the result of WTAP suppressing the transcriptional activity of WT1 in SMCs. WTAP may thus also play a role in messenger RNA processing in mammalian cells, either dependent on or independent of its interaction with WT1.

Synonym: Mum2

Molecular Weight: 45 kDa