XIAP Protein (AVI tag)

Catalog No. ABIN7317819

Product Details

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This XIAP protein is labelled with AVI tag.

Purpose: Recombinant Human XIAP/BIRC4 Protein (AVI Tag)(Active)

Sequence: Leu 121-Thr 356

Characteristics: A DNA sequence encoding the human XIAP (NP_001158.2) (Leu 121-Thr 356) was fused with an AVI tag at the C-terminus, and additonal two amino acids (Gly & Pro) at the N-terminus.

Purity: > 75 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized recombinant human SMAC-His at 10 μg/ml (100 μl/well) can bind recombinant human XIAP-AVI with a linear range of 0.125-1.0 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 25 mM Tris, 10 mM DTT, 1 % glycerol, 0.2M Glutamine Potassium, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Alternative Name: XIAP/BIRC4 (XIAP Products)

Synonyms: API3 Protein, BIRC4 Protein, IAP-3 Protein, ILP1 Protein, MIHA Protein, XLP2 Protein, hIAP-3 Protein, hIAP3 Protein, api3 Protein, birc4 Protein, ilp1 Protein, miha Protein, xlp2 Protein, xxiap Protein, 1110015C02Rik Protein, Aipa Protein, Api3 Protein, Birc4 Protein, IAP3 Protein, ILP-1 Protein, mXIAP Protein, riap3 Protein, fb73d01 Protein, wu:fb73d01 Protein, zgc:91873 Protein, BIRC8 Protein, X-linked inhibitor of apoptosis Protein, X-linked inhibitor of apoptosis L homeolog Protein, XIAP Protein, xiap Protein, xiap.L Protein, Xiap Protein

Background:

Background: E3 ubiquitin-protein ligase XIAP / BIRC4, also known as inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis protein, and IAP-like protein, is a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. XIAP / BIRC4 functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. XIAP / BIRC4 also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this encoding gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Thought to be the most potent apoptosis suppressor, XIAP / BIRC4, directly binds and inhibits caspases -3, -7 and -9. Survivin, which also binds to several caspases, is up-regulated in a many tumour cell types. Defects in XIAP / BIRC4 are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2). XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.

Synonym: API3,BIRC4,hIAP-3,hIAP3,IAP-3,ILP1,MIHA,XLP2

Molecular Weight: 29.1 kDa

NCBI Accession: NP_001158

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis