XPNPEP2 Protein (His tag)
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- Target See all XPNPEP2 Proteins
- XPNPEP2 (X-Prolyl Aminopeptidase (Aminopeptidase P) 2, Membrane-Bound (XPNPEP2))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This XPNPEP2 protein is labelled with His tag.
- Purpose
- Recombinant Human XPNPEP2 Protein (His Tag)(Active)
- Sequence
- Met 1-Ala 650
- Characteristics
- A DNA sequence encoding the human XPNPEP2 (O43895) (Met 1-Ala 650) without the pro peptide was expressed, with a polyhistidine tag at the C-terminus.
- Purity
- > 97 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave the fluorogenic peptide substrate, H-Lys(2-Aminobenzoyl)Pro-Pro-pNitroanilide(K(Abz)PP-pNA).The specific activity is > 300 pmoles/min/μg.
- Top Product
- Discover our top product XPNPEP2 Protein
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X-Prolyl Aminopeptidase (Aminopeptidase P) 2, Membrane-Bound (XPNPEP2) (AA 23-650) protein (His tag)
XPNPEP2 Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
X-Prolyl Aminopeptidase (Aminopeptidase P) 2, Membrane-Bound (XPNPEP2) (AA 22-649) protein (His tag)Crystallography grade XPNPEP2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
X-Prolyl Aminopeptidase (Aminopeptidase P) 2, Membrane-Bound (XPNPEP2) protein (Myc-DYKDDDDK Tag)XPNPEP2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- XPNPEP2 (X-Prolyl Aminopeptidase (Aminopeptidase P) 2, Membrane-Bound (XPNPEP2))
- Alternative Name
- XPNPEP2 (XPNPEP2 Products)
- Synonyms
- APP2 Protein, 9030008G12Rik Protein, mAPP Protein, XPNPEP2 Protein, zK61O11.1 Protein, zgc:63528 Protein, X-prolyl aminopeptidase 2 Protein, X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound Protein, X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound L homeolog Protein, XPNPEP2 Protein, Xpnpep2 Protein, xpnpep2.L Protein, xpnpep2 Protein
- Background
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Background: Aminopeptidase P (APP) is a hydrolase specific for N-terminal imido bonds; which are common to several collagen degradation products; neuropeptides; vasoactive peptides; and cytokines. A membrane-bound and soluble form of this enzyme (XPNPEP2) have been identified as products of two separate genes. XPNPEP2; the X-linked gene that encodes membranous aminopeptidase P (APP); has been reported to associate with APP activity. The membrane aminopeptidase P (XPNPEP2) is largely limited in distribution to endothelia and brush border epithelia. APP and XPNPEP2 contain homologous blocks of sequence common to members of the "pita bread-fold" protein family; of which Escherichia coli methionine aminopeptidase is the prototype. The C-2399A variant in XPNPEP2 is associated with reduced APP activity and a higher incidence of AE-ACEi. XPNPEP2 mRNA was detected in fibroblasts that carry the translocation; suggesting that this gene at least partially escapes X inactivation. XPNPEP2 is a candidate gene for premature ovarian failure (POF).
Synonym: Xaa-Pro Aminopeptidase 2; Aminoacylproline Aminopeptidase; Membrane-Bound Aminopeptidase P; Membrane-Bound APP; Membrane-Bound AmP; mAmP; X-Pro Aminopeptidase 2; XPNPEP2
- Molecular Weight
- 72 kDa
- UniProt
- O43895
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