2B4 Protein (AA 22-221) (Biotin,His-Avi Tag)

Catalog No. ABIN7273782

This Recombinant 2B4 protein is produced in HEK-293 Cells.

Quick Overview for 2B4 Protein (AA 22-221) (Biotin,His-Avi Tag) (ABIN7273782)

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-221

Purification tag / Conjugate: This 2B4 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human 2B4/CD244/SLAMF4 Protein

Sequence: Cys22-Arg221

Characteristics: Recombinant Biotinylated Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys22-Arg221.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human 2B4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).,Biotinylated Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human CD48, hFc Tag on ProA-Biosensor can bind Biotinylated Human 2B4, His-Avi Tag with an affinity constant of 24.90 nM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated Human 2B4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: 2B4

Background: 2B4 (CD244) is expressed by memory-phenotype CD8(+) T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.

Molecular Weight: 24.9 kDa. Due to glycosylation, the protein migrates to 48-70 kDa based on Bis-Tris PAGE result.

UniProt: Q9BZW8