B7-H6 Protein (AA 25-262) (Fc Tag)

Catalog No. ABIN7273963

Product Details

Target: B7-H6 (NCR3LG1) (Natural Killer Cell Cytotoxicity Receptor 3 Ligand 1 (NCR3LG1))

Protein Type: Recombinant

Protein Characteristics: AA 25-262

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This B7-H6 protein is labelled with Fc Tag.

Purpose: Human B7-H6/NCR3LG1 Protein

Sequence: Asp25-Ser262

Characteristics: Recombinant Human B7-H6/NCR3LG1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp25-Ser262.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.292 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: B7-H6 (NCR3LG1) (Natural Killer Cell Cytotoxicity Receptor 3 Ligand 1 (NCR3LG1))

Alternative Name: B7-H6 (NCR3LG1 Products)

Synonyms: B7-H6 Protein, B7H6 Protein, DKFZp686O24166 Protein, natural killer cell cytotoxicity receptor 3 ligand 1 Protein, NCR3LG1 Protein

Background: B7-H6 is a glycosylated member of the B7 family of immune co-stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.

Molecular Weight: 53.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.