VTCN1 Protein (AA 33-194) (hFc Tag)
Quick Overview for VTCN1 Protein (AA 33-194) (hFc Tag) (ABIN7273974)
Target
See all VTCN1 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 33-194
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Purification tag / Conjugate
- This VTCN1 protein is labelled with hFc Tag.
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Purpose
- Mouse B7-H5/Gi24/VISTA Protein
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Sequence
- Phe33-Ala194
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Characteristics
- Recombinant Mouse B7-H5/Gi24/VISTA Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Phe33-Ala194.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse B7-H5 is greater than 95% as determined by SEC-HPLC.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
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Alternative Name
- B7-H5
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Background
- B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.
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Molecular Weight
- 44.77 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result.
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NCBI Accession
- NP_083008
Target
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