BST1 Protein (AA 29-293) (His tag)

Catalog No. ABIN7273991

Product Details

Target: BST1 (Bone Marrow Stromal Cell Antigen 1 (BST1))

Protein Type: Recombinant

Protein Characteristics: AA 29-293

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This BST1 protein is labelled with His tag.

Purpose: Human BST1 Protein

Sequence: Gly29-Ala293

Characteristics: Recombinant Human BST1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly29-Ala293.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human BST1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BST1 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BST1 (Bone Marrow Stromal Cell Antigen 1 (BST1))

Alternative Name: BST1 (BST1 Products)

Synonyms: BST1 Protein, 114/A10 Protein, A530073F09 Protein, BP-3 Protein, Bp3 Protein, Bsta1 Protein, CD157 Protein, Ly65 Protein, bone marrow stromal cell antigen 1 Protein, BST1 Protein, Bst1 Protein

Background: BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism.

Molecular Weight: 31.2 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.