CD21 Protein (AA 21-971) (His tag)

Catalog No. ABIN7274107

Product Details

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Protein Type: Recombinant

Protein Characteristics: AA 21-971

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD21 protein is labelled with His tag.

Purpose: Human CD21 Protein

Sequence: Ile21-Arg971

Characteristics: Recombinant Human CD21 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Arg971.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD21, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD21 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CD21 (CR2 Products)

Synonyms: C3DR Protein, CD21 Protein, CR Protein, CVID7 Protein, SLEB9 Protein, Cd21 Protein, cd21 Protein, CR1 Protein, CR1-L Protein, CR2 Protein, complement C3d receptor 2 Protein, complement C3b/C4b receptor 1 (Knops blood group) Protein, CR2 Protein, Cr2 Protein, CR1 Protein

Background: CD21 mRNA expression, an EBV-entry receptor, was transiently detected in NK cells after exosome treatment, and levels decreased after further culture. CD21 protein expression was also transiently transferred to NK cells after co-culture with an EBV-positive Burkitt lymphoma cell line (Raji) via trogocytosis. However, EBV did not infect NK cells through CD21-mediated trogocytosis.

Molecular Weight: 105.3 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.

Pathways: Complement System