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CD40 Protein (CD40) (AA 24-193) (His tag)

CD40 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7274188
  • Target See all CD40 Proteins
    CD40
    Protein Type
    Recombinant
    Protein Characteristics
    AA 24-193
    Origin
    • 25
    • 15
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 32
    • 8
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This CD40 protein is labelled with His tag.
    Purpose
    Mouse CD40/TNFRSF5 Protein
    Sequence
    Val24-Arg193
    Characteristics
    Recombinant Mouse CD40/TNFRSF5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Arg193.
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 78.3ng/ml determined by ELISA. See testing image for detail.
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    Discover our top product CD40 Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    CD40
    Alternative Name
    CD40 (CD40 Products)
    Synonyms
    Bp50 Protein, CDW40 Protein, TNFRSF5 Protein, p50 Protein, AI326936 Protein, GP39 Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TRAP Protein, Tnfrsf5 Protein, TNFSF5 Protein, CD40 molecule Protein, CD40 antigen Protein, CD40 Protein, Cd40 Protein
    Background
    CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 Molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
    Molecular Weight
    19.9 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
    Pathways
    NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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