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FCGR3A Protein (His-Avi Tag,Biotin)

FCGR3A Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Catalog No. ABIN7274282
  • Target See all FCGR3A Proteins
    FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
    Protein Type
    Recombinant
    Origin
    • 21
    • 3
    • 3
    • 1
    Human
    Source
    • 15
    • 6
    • 3
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This FCGR3A protein is labelled with His-Avi Tag,Biotin.
    Sequence
    Gly17-Gln208(F176)
    Purity
    > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA. The affinity constant of 0.14μM as determined in SPR assay (Biacore T200). See testing image for detail.
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    Discover our top product FCGR3A Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
    Storage
    4 °C,-80 °C
    Storage Comment
    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))
    Alternative Name
    Fc gamma RIIIA (FCGR3A Products)
    Synonyms
    CD16 Protein, CD16A Protein, FCG3 Protein, FCGR3 Protein, FCGRIII Protein, FCR-10 Protein, FCRIII Protein, FCRIIIA Protein, IGFR3 Protein, Fcgr4 Protein, Fc fragment of IgG receptor IIIa Protein, FCGR3A Protein, Fcgr3a Protein
    Background
    CD16FCRIIIA, Fc fragment of IgG, Fc gamma RIIIA, Fc-gamma RIII-alpha, FCGR3, FCGR3A, FCGRIII, FcgRIIIA, FcR-10, CD16A, FCG3, IGFR3, FcRIIIa, FcRIII, CD16, IMD20,Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
    Molecular Weight
    24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
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