CHRDL1 Protein (AA 28-456) (His tag)
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- Target See all CHRDL1 Proteins
- CHRDL1 (Chordin-Like 1 (CHRDL1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 28-456
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CHRDL1 protein is labelled with His tag.
- Purpose
- Human CHRDL1 Protein
- Sequence
- Glu28-Cys456
- Characteristics
- Recombinant Human CHRDL1 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Glu28-Cys456.
- Purity
- > 90 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CHRDL1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CHRDL1 Antibody, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA. See testing image for detail.
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Chordin-Like 1 (CHRDL1) (AA 1-450) protein (GST tag)
CHRDL1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Chordin-Like 1 (CHRDL1) (AA 347-456) protein (GST tag)CHRDL1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Chordin-Like 1 (CHRDL1) (AA 23-447) protein (His tag)Crystallography grade CHRDL1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Chordin-Like 1 (CHRDL1) (AA 22-450) protein (His tag)Crystallography grade CHRDL1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 4 mM HCl.
- Buffer
- Lyophilized from 0.22μm filtered solution in 4 mM HCl. Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CHRDL1 (Chordin-Like 1 (CHRDL1))
- Alternative Name
- CHRDL1 (CHRDL1 Products)
- Synonyms
- CHL Protein, NRLN1 Protein, VOPT Protein, dA141H5.1 Protein, CHL1 Protein, Nrln1 Protein, chl Protein, vopt Protein, nrln1 Protein, da141h5.1 Protein, Kjr Protein, Neurogenesin-1 Protein, Ng1 Protein, chordin like 1 Protein, chordin-like 1 Protein, CHRDL1 Protein, Chrdl1 Protein, chrdl1 Protein
- Background
- CHRDL1 (Chordin-like 1) is a secreted protein that acts as an antagonist of bone morphogenetic protein (BMP). BMP plays a role as an activator of BMP receptor II (BMPR II), which mediates extracellular to intracellular signal transmission and is involved in carcinogenesis and metastasis.The hypermethylation of the CHRDL1 promoter in gastric cancer, which induced low expression of CHRDL1 and decreased its secretion to the supernatant.
- Molecular Weight
- 50 kDa same as Tris-Bis PAGE result.
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