CEACAM8 Protein (AA 35-319) (His tag)

Catalog No. ABIN7274330

Product Details

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Protein Type: Recombinant

Protein Characteristics: AA 35-319

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CEACAM8 protein is labelled with His tag.

Purpose: Human CEACAM-8/CD66b Protein

Sequence: Gln35-Ser319

Characteristics: Recombinant Human CEACAM-8/CD66b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln35-Ser319.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 4.59 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Alternative Name: CEACAM-8 (CEACAM8 Products)

Synonyms: CD67 Protein, CGM6 Protein, CD66b Protein, NCA-95 Protein, CEACAM8 Protein, CEACAM1 Protein, MGC142545 Protein, cd67 Protein, cgm6 Protein, cd66b Protein, nca-95 Protein, carcinoembryonic antigen related cell adhesion molecule 8 Protein, carcinoembryonic antigen related cell adhesion molecule 1 Protein, carcinoembryonic antigen-related cell adhesion molecule 8 Protein, CEACAM8 Protein, CEACAM1 Protein, LOC612469 Protein, ceacam8 Protein

Background: Eosinophils and their products are likely important in the pathophysiology of allergic diseases, such as bronchial asthma, and in host immunity to parasitic organisms. CD66b (CEACAM8, CGM6, NCA-95) is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic Ag supergene family. CD66b is an activation marker for human granulocytes.

Molecular Weight: 32.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

NCBI Accession: NP_001807