CLEC12A Protein (AA 65-265) (Fc-Avi Tag,Biotin)

Catalog No. ABIN7274359

Product Details

Target: CLEC12A (C-Type Lectin Domain Family 12, Member A (CLEC12A))

Protein Type: Recombinant

Protein Characteristics: AA 65-265

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CLEC12A protein is labelled with Fc-Avi Tag,Biotin.

Purpose: Biotinylated Human CLEC12A/MICL/CLL-1 Protein

Sequence: His65-Ala265

Characteristics: Recombinant Biotinylated Human CLEC12A/MICL/CLL-1 Protein is expressed from HEK293 with hFc tag and Avi tag at the N-Terminus.It contains His65-Ala265.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CLEC12A Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human CLEC12A, hFc Tag with the EC50 of 30.7ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CLEC12A (C-Type Lectin Domain Family 12, Member A (CLEC12A))

Alternative Name: CLEC12A (CLEC12A Products)

Synonyms: MGC179455 Protein, CLL-1 Protein, CLL1 Protein, DCAL-2 Protein, MICL Protein, D230024O04 Protein, KLRL1 Protein, Micl Protein, C-type lectin domain family 12 member A Protein, C-type lectin domain family 12, member a Protein, C-type lectin domain family 12, member A Protein, CLEC12A Protein, Clec12a Protein

Background: CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML.

Molecular Weight: 51.7 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Tris-Bis PAGE result.