SARS-CoV-2 Spike Protein (RBD) (His-Avi Tag)

Catalog No. ABIN7274399

Recombinant SARS-CoV-2 Spike protein expressed in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike Protein (RBD) (His-Avi Tag) (ABIN7274399)

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: RBD

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with His-Avi Tag.

Purpose: SARS-COV-2 Spike RBD Protein

Sequence: Arg319-Asn532

Characteristics: Recombinant SARS-COV-2 Spike RBD Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg319-Asn532.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-COV-2 Spike RBD, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA. The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike

Background: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 27 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.