CTLA4 Protein (AA 36-161) (Fc Tag)

Catalog No. ABIN7274436

This Recombinant CTLA4 protein is produced in HEK-293 Cells.

Quick Overview for CTLA4 Protein (AA 36-161) (Fc Tag) (ABIN7274436)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 36-161

Purification tag / Conjugate: This CTLA4 protein is labelled with Fc Tag.

Purpose: Human CTLA-4/CD152 Protein

Sequence: Lys36-Asp161

Characteristics: Recombinant Human CTLA-4/CD152 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Lys36-Asp161.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human B7-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA-4

Background: CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation - a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.

Molecular Weight: 40.1 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.

UniProt: P16410-1

Pathways: Cancer Immune Checkpoints