FASL Protein (His tag)
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- Target See all FASL Proteins
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FASL protein is labelled with His tag.
- Sequence
- Pro134-Leu281
- Purity
- > 95% as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Fas Ligand, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 134-281) (Active) protein (His tag)
FASL Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) protein (GST tag)FASL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 172-281) protein (GST tag)FASL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 101-279) protein (His tag)Crystallography grade FASL Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) protein (Strep Tag)Crystallography grade FASL Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-279) protein (Strep Tag)Crystallography grade FASL Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-81) protein (rho-1D4 tag)Crystallography grade FASL Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 130-281) protein (rho-1D4 tag)Crystallography grade FASL Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-129) protein (rho-1D4 tag)Crystallography grade FASL Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-127) protein (rho-1D4 tag)Crystallography grade FASL Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
- Alternative Name
- Fas Ligand (FASL Products)
- Synonyms
- ALPS1B Protein, APT1LG1 Protein, APTL Protein, CD178 Protein, CD95-L Protein, CD95L Protein, FASL Protein, TNFSF6 Protein, fasl Protein, Fas-L Protein, Faslg Protein, Tnfsf6 Protein, gld Protein, Apt1Lg1 Protein, Fasl Protein, FASLG Protein, zgc:162027 Protein, Fas ligand Protein, Fas ligand L homeolog Protein, Fas ligand (TNF superfamily, member 6) Protein, FASLG Protein, faslg.L Protein, Fasl Protein, Faslg Protein, faslg Protein
- Background
- APTL, CD95-L, Fas ligand, APT1LG1, TNFSF6, FASLG, ALPS1B, CD178, FASL, TNLG1A,Fas ligand (FasL, CD95L) is a 40- kDa type II transmembrane protein that binds to Fas (CD95) receptors and promotes programmed cell death. Fas receptors are expressed at higher levels in many tumors than in normal cells.Somewhat paradoxically, elimination of Fas or FasL from tumors also leads to death induced by CD95 receptor/ligand elimination (DICE).
- Molecular Weight
- 18 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.
- Pathways
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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