TNFSF15 Protein (Trimer) (His-DYKDDDDK Tag)

Catalog No. ABIN7274680

Product Details

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Protein Type: Recombinant

Protein Characteristics: Trimer

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFSF15 protein is labelled with His-DYKDDDDK Tag.

Sequence: Asp91-Leu251

Purity: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TNFSF15 Trimer, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: 4 °C,-80 °C

Storage Comment: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFSF15 (Tumor Necrosis Factor (Ligand) Superfamily, Member 15 (TNFSF15))

Alternative Name: TNFSF15 (TNFSF15 Products)

Synonyms: TTPAL Protein, TL1 Protein, TL1A Protein, VEGI Protein, VEGI192A Protein, Tl1 Protein, Tl1a Protein, Vegi Protein, tumor necrosis factor superfamily member 15 Protein, TNF superfamily member 15 Protein, tumor necrosis factor (ligand) superfamily, member 15 Protein, TNFSF15 Protein, Tnfsf15 Protein

Background: TL1A, VEGI-251, TNFSF15, TL1, VEGI, VEGI192A,TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

Molecular Weight: 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

UniProt: O95150

Pathways: Positive Regulation of Endopeptidase Activity, Autophagy