TNFSF18 Protein (Trimer) (His-DYKDDDDK Tag,Biotin)
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- Target See all TNFSF18 Proteins
- TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))
- Protein Type
- Recombinant
- Protein Characteristics
- Trimer
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TNFSF18 protein is labelled with His-DYKDDDDK Tag,Biotin.
- Purpose
- Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling)
- Sequence
- Gln50-Ser177
- Characteristics
- Recombinant Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and flag tag at the N-Terminus.It contains Gln50-Ser177.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GITR, His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2μg/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))
- Alternative Name
- GITR Ligand (TNFSF18 Products)
- Synonyms
- Gitrl Protein, AITRL Protein, GITRL Protein, TL6 Protein, hGITRL Protein, TNLG2A Protein, tumor necrosis factor (ligand) superfamily, member 18 Protein, TNF superfamily member 18 Protein, Tnfsf18 Protein, TNFSF18 Protein
- Background
- Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.
- Molecular Weight
- 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9UNG2
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