GAS6 Protein (AA 49-678) (His tag)

Catalog No. ABIN7274698

This Recombinant GAS6 protein is expressed in HEK-293 Cells.

Quick Overview for GAS6 Protein (AA 49-678) (His tag) (ABIN7274698)

Target: GAS6 (Growth Arrest-Specific 6 (GAS6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 49-678

Purification tag / Conjugate: This GAS6 protein is labelled with His tag.

Purpose: Human GAS6 Protein

Sequence: Ala49-Ala678

Characteristics: Recombinant Human GAS6 Protein is expressed from HEK293 with His tag at the C-Terminus., It contains Ala49-Ala678.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human GAS6, His Tag immobilized on CM5 Chip can bind Human TYRO3, His Tag with an affinity constant of 2.36 uM as determined in SPR assay (Biacore T200).,Immobilized Human GAS6, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human Axl, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA (QC Test).,Immobilized Human MERTK, mFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Human GAS6, His Tag with the EC50 of 3.55ug/ml determined by ELISA.,Immobilized Human GAS6, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GAS6 Antibody, hFc Tag with the EC50 of 12.3 ng/ml determined by ELISA.,Human GAS6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human GAS6 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 500 mM NaCl ( pH 8.5). Normally 8 % mannitol is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GAS6 (Growth Arrest-Specific 6 (GAS6))

Alternative Name: GAS6

Background: Growth arrest-specific 6, also known as Gas6, is a human gene encoding the Gas6 protein, which was originally found to be upregulated in growth-arrested fibroblasts. Gas6 is a member of the vitamin K-dependent family of proteins expressed in many human tissues and regulates several biological processes in cells, including proliferation, survival and migration, by binding to its receptors Tyro3, Axl and Mer (TAM).

Molecular Weight: 70.60 kDa. Due to glycosylation, the protein migrates to 71-75 kDa based on Bis-Tris PAGE result.

UniProt: Q14393

Pathways: RTK Signaling, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response