GDF15 Protein (AA 189-303) (His tag)

Catalog No. ABIN7274728

This Recombinant GDF15 protein is expressed in Escherichia coli (E. coli).

Quick Overview for GDF15 Protein (AA 189-303) (His tag) (ABIN7274728)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 189-303

Purification tag / Conjugate: This GDF15 protein is labelled with His tag.

Purpose: Mouse GDF15 Protein

Sequence: Ser189-Ala303

Characteristics: Recombinant Mouse GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ser189-Ala303.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Mouse GDF15, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.68μg/ml determined by ELISA. The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM HAc ( pH 2.9).

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM HAc ( pH 2.9). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15

Background: Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.

Molecular Weight: 13.78 kDa. The protein migrates to 15-16 kDa based on Tris-Bis PAGE result.

UniProt: Q9Z0J7

Pathways: SARS-CoV-2 Protein Interactome