CD42b Protein (AA 17-531) (His tag)
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- Target See all CD42b (GP1BA) Proteins
- CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 17-531
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD42b protein is labelled with His tag.
- Purpose
- Human CD42b/GP1BA Protein
- Sequence
- His17-Leu531
- Characteristics
- Recombinant Human CD42b/GP1BA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His17-Leu531.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD42b, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD42b Antibody, hFc Tag with the EC50 of 8.9ng/ml determined by ELISA. See testing image for detail.
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Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA) (AA 1-626) protein (GST tag)
GP1BA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA) (AA 19-128) protein (GST tag)GP1BA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA) (AA 17-306) protein (His tag)GP1BA Origin: Human Host: HEK-293T Cells Recombinant SDS
Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA) (AA 634-734) protein (His tag)Crystallography grade GP1BA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA) (AA 17-531) protein (His tag)Crystallography grade GP1BA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA) (AA 17-612) protein (His tag)Crystallography grade GP1BA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA) (AA 17-652) protein (rho-1D4 tag)Crystallography grade GP1BA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA) (AA 17-734) protein (rho-1D4 tag)Crystallography grade GP1BA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA) (AA 19-291) protein (T7 tag,His tag)GP1BA Origin: Human Host: Escherichia coli (E. coli) Recombinant ≥ 90 % SDS, ELISA, WB, IP
Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA) (AA 17-282) protein (S tag,His tag)GP1BA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant ≥ 92 % SDS, ELISA, WB, IP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))
- Alternative Name
- CD42b (GP1BA Products)
- Synonyms
- BDPLT1 Protein, BDPLT3 Protein, BSS Protein, CD42B Protein, CD42b-alpha Protein, DBPLT3 Protein, GP1B Protein, GPIbA Protein, VWDP Protein, GP1BA Protein, GPIba Protein, GPIbalpha Protein, glycoprotein Ib platelet alpha subunit Protein, glycoprotein Ib (platelet), alpha polypeptide Protein, glycoprotein 1b, alpha polypeptide Protein, GP1BA Protein, Gp1ba Protein
- Background
- Absence of the CD42 complex leads to the Bernard-Soulier syndrome (BSS), which is a bleeding disorder characterized by thrombocytopenia and giant platelets. So far, mutations have been found in the genes encoding CD42a, CD42b and CD42c in patients with BSS**.
- Molecular Weight
- 57.6 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.
- UniProt
- P07359
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