ErbB2/Her2 Protein (AA 23-652) (His-Avi Tag)

Catalog No. ABIN7274799

This Recombinant ErbB2/Her2 protein is produced in HEK-293 Cells.

Quick Overview for ErbB2/Her2 Protein (AA 23-652) (His-Avi Tag) (ABIN7274799)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-652

Purification tag / Conjugate: This ErbB2/Her2 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human Her2/ErbB2 Protein

Sequence: Thr23-Thr652

Characteristics: Recombinant Human Her2/ErbB2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr23-Thr652.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).,Human Her2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-HER2 Antibody on ProA-Biosensor can bind Human Her2, His Tag with an affinity constant of 32 nM as determined in BLI assay (Gator® Prime).,The purity of Human Her2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: Her2

Background: ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane.

Molecular Weight: 72.3 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.

UniProt: P04626

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development