IDH1 Protein (AA 2-414) (His tag)
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- Target See all IDH1 Proteins
- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-414
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IDH1 protein is labelled with His tag.
- Purpose
- Human IDH1 Protein
- Sequence
- Ser2-Leu414
- Characteristics
- Recombinant Human IDH1 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Ser2-Leu414.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 341-414) protein (His-IF2DI Tag)
IDH1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 1-414) protein (His tag)IDH1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 1-414) protein (GST tag)IDH1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 2-414) protein (His tag)Crystallography grade IDH1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as 0.22μm filtered solution in 50 mM Tris, 200 mM NaCl, 1 mM DTT, 10 % Glycerol ( pH 7.5).
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -80 °C
- Storage Comment
- Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
- Alternative Name
- IDH1 (IDH1 Products)
- Synonyms
- IDCD Protein, IDH Protein, IDP Protein, IDPC Protein, PICD Protein, NADP-CICDH Protein, AI314845 Protein, AI788952 Protein, E030024J03Rik Protein, Id-1 Protein, Idh-1 Protein, Idpc Protein, cb876 Protein, fm90e09 Protein, im:7143416 Protein, wu:fm90e09 Protein, F23E12.180 Protein, F23E12_180 Protein, IDH-I Protein, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1 Protein, isocitrate dehydrogenase 1 Protein, isocitrate dehydrogenase I Protein, isocitrate dehydrogenase (NADP(+)) 1, cytosolic Protein, isocitrate dehydrogenase 1 (NADP+), soluble Protein, isocitrate dehydrogenase 1 (NADP+) L homeolog Protein, isocitrate dehydrogenase 1 Protein, IDH1 Protein, Idh1 Protein, idh1.L Protein, idh1 Protein
- Background
- Isocitrate dehydrogenase (IDH) is a metabolic enzyme that converts isocitrate to α-ketoglutarate (α-KG). Genetic gain-of-function mutations in IDH1 and IDH2 confer a neomorphic activity that allow reduction of α -KG to (R)-2- hydroxyglutarate, the accumulation of which results in the development of cancers like low grade gliomas and leukemia.Isocitrate dehydrogenase 1 (IDH1) is the most frequently mutated metabolic gene in cancer.
- Molecular Weight
- 47.35 kDa same as Tris-Bis PAGE result.
- UniProt
- O75874
- Pathways
- Warburg Effect
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