IFNA Protein (AA 27-243) (His tag)

Catalog No. ABIN7274844

This Recombinant IFNA protein is produced in HEK-293 Cells.

Quick Overview for IFNA Protein (AA 27-243) (His tag) (ABIN7274844)

Target: IFNA (Interferon alpha (IFNA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-243

Purification tag / Conjugate: This IFNA protein is labelled with His tag.

Purpose: Human IFN alpha/beta R2 Protein

Sequence: Ile27-Lys243

Characteristics: Recombinant Human IFN alpha/beta R2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile27-Lys243.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200).,Human IFN alpha/beta R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human IFN alpha/beta R2, His Tag on Anti-His-Biosensor, can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.10 nM as determined in BLI assay (Gator).,The purity of Human IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IFNA (Interferon alpha (IFNA))

Alternative Name: IFN alpha

Background: Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little is known about IFN-α-regulated miRNAs in DCs. Since several miRNAs are involved in regulating DC functions, it is important to investigate whether IFN-α's effects on DCs are mediated through miRNAs as well.

Molecular Weight: 25.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: P48551

Pathways: JAK-STAT Signaling, TLR Signaling, Hepatitis C, Inflammasome