IFNA Protein (AA 27-429) (His-Avi Tag)

Catalog No. ABIN7274853

This Recombinant IFNA protein is produced in HEK-293 Cells.

Quick Overview for IFNA Protein (AA 27-429) (His-Avi Tag) (ABIN7274853)

Target: IFNA (Interferon alpha (IFNA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-429

Purification tag / Conjugate: This IFNA protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Mouse IFN alpha/beta R1 Protein

Sequence: Glu27-Thr429

Characteristics: Recombinant Mouse IFN alpha/beta R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu27-Thr429.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 3.61 nM as determined in SPR assay (Biacore T200).,Mouse IFN alpha/beta R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Mouse IFN alpha/beta R1, His-Avi Tag on Anti-His-Biosensor can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.81 nM as determined in BLI assay (Gator® Prime).,The purity of Mouse IFN alpha/beta R1 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IFNA (Interferon alpha (IFNA))

Alternative Name: IFN alpha

Background: IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions.Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves.

Molecular Weight: 46.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

UniProt: P33896

Pathways: JAK-STAT Signaling, TLR Signaling, Hepatitis C, Inflammasome