IL12 Protein (AA 23-328) (His-Avi Tag)

Catalog No. ABIN7274898

This Recombinant IL12 protein is produced in HEK-293 Cells.

Quick Overview for IL12 Protein (AA 23-328) (His-Avi Tag) (ABIN7274898)

Target: IL12 (Interleukin 12 (IL12))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-328

Purification tag / Conjugate: This IL12 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human IL-12 Protein

Sequence: Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)

Characteristics: Recombinant Human IL-12 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of IL-12A.It contains Ile23-Ser328 (IL-12B) & Arg23-Ser219 (IL-12A).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-12, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test).,Human IL-12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50-for this effect is 0.1 - 1 ng/mL.,The purity of Human IL-12 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12

Background: Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.

Molecular Weight: 34.7 kDa(IL-12B) & 25.5 kDa(IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Bis-Tris PAGE result.

UniProt: P29460

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome