IL-31 Protein (AA 24-164) (His tag)
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- Target See all IL-31 (IL31) Proteins
- IL-31 (IL31) (Interleukin 31 (IL31))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-164
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL-31 protein is labelled with His tag.
- Purpose
- Human IL-31 Protein
- Sequence
- Ser24-Thr164
- Characteristics
- Recombinant Human IL-31 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ser24-Thr164.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-31, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.2μg/ml determined by ELISA. The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 31 (IL31) (AA 27-163) protein (His tag)
IL31 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 31 (IL31) (AA 24-163) protein (His tag)IL31 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 31 (IL31) (AA 24-163) protein (His tag)Crystallography grade IL31 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 31 (IL31) (AA 24-164) (Active) protein (His tag)IL31 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL-31 (IL31) (Interleukin 31 (IL31))
- Alternative Name
- IL-31 (IL31 Products)
- Synonyms
- IL-31 Protein, 1700013B14Rik Protein, IL31 Protein, interleukin 31 Protein, IL31 Protein, Il31 Protein
- Background
- Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells.
- Molecular Weight
- 16.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
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