IL15RA Protein (AA 31-205) (Biotin,His-Avi Tag)
Quick Overview for IL15RA Protein (AA 31-205) (Biotin,His-Avi Tag) (ABIN7274957)
Target
See all IL15RA ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 31-205
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Purification tag / Conjugate
- This IL15RA protein is labelled with Biotin,His-Avi Tag.
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Purpose
- Biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein
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Sequence
- Ile31-Thr205
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Characteristics
- Recombinant Biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile31-Thr205.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human IL-15 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-15RA, His Tag with the EC50 of 55.8ng/ml determined by ELISA.,Biotinylated Human IL-15RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human IL-15RA is greater than 95% as determined by SEC-HPLC.
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Interleukin 15 Receptor, alpha (IL15RA) (AA 31-205) (Active) protein (His-Avi Tag)
IL15RA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 15 Receptor, alpha (IL15RA) (Active) protein (Fc Tag)IL15RA Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by SDS-PAGE Active
Interleukin 15 Receptor, alpha (IL15RA) (AA 31-205) (Active) protein (Fc Tag,AVI tag,Biotin)IL15RA Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 Receptor, alpha (IL15RA) (AA 31-130) protein (GST tag)IL15RA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 15 Receptor, alpha (IL15RA) (AA 31-205) protein (Fc Tag)IL15RA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Interleukin 15 Receptor, alpha (IL15RA) (AA 31-205) (Active) protein (hIgG-His-tag)IL15RA Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS, AcA Active
Interleukin 15 Receptor, alpha (IL15RA) (AA 31-205) (Active) protein (Fc Tag)IL15RA Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- IL15RA (Interleukin 15 Receptor, alpha (IL15RA))
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Alternative Name
- IL-15RA
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Background
- Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
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Molecular Weight
- 21.3 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
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UniProt
- Q13261
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Pathways
- JAK-STAT Signaling
Target
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