IL-2 R beta & IL-2 R gamma (AA 27-240) (Active) protein (hFc Tag)

Catalog No. ABIN7275026

Recombinant IL-2 R beta & IL-2 R gamma protein expressed in HEK-293 Cells.

Quick Overview for IL-2 R beta & IL-2 R gamma (AA 27-240) (Active) protein (hFc Tag) (ABIN7275026)

Target: IL-2 R beta & IL-2 R gamma

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 27-240

Purification tag / Conjugate: hFc Tag

Purpose: Human IL-2 R beta&IL-2 R gamma Protein

Sequence: Ala27-Thr240(IL-2 R beta) & Leu23-Ala262(IL-2 R gamma)

Specificity: Uni-Prot: P14784 (IL-2 R beta), P31785-1 (IL-2 R gamma)

Characteristics: Recombinant Human IL-2 R beta&IL-2 R gamma Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus of IL-2 R beta and IL-2 R gamma.It contains Ala27-Thr240 (IL-2 R beta) and Leu23-Ala262 (IL-2 R gamma).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).,Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).,Human IL-2 R beta&IL-2 R gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-2 R beta & IL-2 R gamma

Background: Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change.

Molecular Weight: 51.3 kDa (IL-2 R beta) and 54.6 kDa (IL-2 R gamma). Due to glycosylation, the protein migrates to 65-70 kDa and 80-100 kDa based on Bis-Tris PAGE result.

UniProt: P14784