F11R Protein (AA 28-238) (His tag)

Catalog No. ABIN7275080

Product Details

Target: F11R (F11 Receptor (F11R))

Protein Type: Recombinant

Protein Characteristics: AA 28-238

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This F11R protein is labelled with His tag.

Purpose: Human JAM-A Protein

Sequence: Ser28-Val238.

Characteristics: Recombinant Human JAM-A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Val238.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human JAM-A, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-JAM-A Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: F11R (F11 Receptor (F11R))

Alternative Name: JAM-A (F11R Products)

Synonyms: CD321 Protein, JAM Protein, JAM1 Protein, JAMA Protein, JCAM Protein, KAT Protein, PAM-1 Protein, 9130004G24 Protein, AA638916 Protein, ESTM33 Protein, JAM-1 Protein, JAM-A Protein, Jcam Protein, Jcam1 Protein, Ly106 Protein, Jam1 Protein, F11 receptor Protein, F11R Protein, F11r Protein

Background: Junctional adhesion molecule A (JAM-A), a cell adhesion molecule, is highly elevated in human GBM cancer stem cells and predicts poor patient prognosis. While JAM-A is also highly expressed in other cells in the tumor microenvironment, specifically microglia and macrophages,JAM-A functions to suppress pathogenic microglial activation in the female tumor microenvironment, highlighting an emerging role for sex differences in the GBM microenvironment and suggesting that sex differences extend beyond previously reported tumor cell-intrinsic differences.

Molecular Weight: 24 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.

Pathways: Cell-Cell Junction Organization