KLRG1 Protein (AA 60-195) (Fc Tag)

Catalog No. ABIN7275099

Product Details

Target: KLRG1 (Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1))

Protein Type: Recombinant

Protein Characteristics: AA 60-195

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This KLRG1 protein is labelled with Fc Tag.

Purpose: Human KLRG1 Protein

Sequence: Leu60-Phe195

Characteristics: Recombinant Human KLRG1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Leu60-Phe195.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human KLRG1, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-KLRG1 Antibody, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: KLRG1 (Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1))

Alternative Name: KLRG1 (KLRG1 Products)

Synonyms: KLRG1 Protein, 2F1-Ag Protein, MAFA Protein, MAFA-L Protein, 2F1 Protein, CLEC15A Protein, MAFA-2F1 Protein, MAFA-LIKE Protein, killer cell lectin like receptor G1 Protein, killer cell lectin-like receptor subfamily G, member 1 Protein, KLRG1 Protein, Klrg1 Protein

Background: Immune homeostasis requires the tight, tissue-specific control of the different CD4 Foxp3 regulatory T (Treg) cell populations. The cadherin-binding inhibitory receptor killer cell lectin-like receptor G1 (KLRG1) is expressed by a subpopulation of Treg cells with GATA3 effector phenotype.Lack of KLRG1 on Treg cells conferred on them a competitive advantage in the gut, but not in lymphoid organs. Hence, although absence of KLRG1 is not enough to increase intestinal Treg cells in KLRG1 knockout mice, KLRG1 ligation reduces T-cell receptor signals and the competitive fitness of individual Treg cells in the intestine.

Molecular Weight: 42.8 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.