LAIR1 Protein (AA 22-163) (Fc Tag)

Catalog No. ABIN7275117

Product Details

Target: LAIR1 (Leukocyte-Associated Immunoglobulin-Like Receptor 1 (LAIR1))

Protein Type: Recombinant

Protein Characteristics: AA 22-163

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This LAIR1 protein is labelled with Fc Tag.

Purpose: Human LAIR1/CD305 Protein

Sequence: Gln22-His163

Characteristics: Recombinant Human LAIR1/CD305 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-His163.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Collagen I, No Tag at 2μg/ml (50μl/well) on the plate. Dose response curve for Human LAIR1, hFc Tag with the EC50 of 1.17μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LAIR1 (Leukocyte-Associated Immunoglobulin-Like Receptor 1 (LAIR1))

Alternative Name: LAIR1 (LAIR1 Products)

Synonyms: 5133400O11Rik Protein, BB115266 Protein, D7Bwg0421e Protein, Lair-1 Protein, mLair-1 Protein, CD305 Protein, LAIR-1 Protein, leukocyte-associated Ig-like receptor 1 Protein, leukocyte associated immunoglobulin like receptor 1 Protein, leukocyte-associated immunoglobulin-like receptor 1 Protein, Lair1 Protein, LAIR1 Protein

Background: LAIR1 (leukocyte-associated Ig-like receptor-1, designated CD305) is an approximately 40 kDa type I transmembrane inhibitory glycoprotein belonging to the Ig superfamily.LAIR1 functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation.

Molecular Weight: 42.4 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.