LRRC15 Protein (His-Avi Tag)

Catalog No. ABIN7275215

Product Details

Target: LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This LRRC15 protein is labelled with His-Avi Tag.

Sequence: Tyr22-Gly538

Purity: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LRRC15, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LRRC15 Antibody, hFc Tag with the EC₅₀ of 20.4ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Storage: 4 °C,-80 °C

Storage Comment: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))

Alternative Name: LRRC15 (LRRC15 Products)

Synonyms: cb894 Protein, zgc:158286 Protein, LIB Protein, 5430427N11Rik Protein, Lib Protein, leucine rich repeat containing 15 Protein, leucine-rich repeat-containing protein 15 Protein, carboxypeptidase N subunit 2 Protein, LRRC15 Protein, lrrc15 Protein, CpipJ_CPIJ004946 Protein, CpipJ_CPIJ005191 Protein, CpipJ_CPIJ007745 Protein, CPN2 Protein, Lrrc15 Protein

Background: LIB, LRRC15,The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.  LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta, small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.

Molecular Weight: 60.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.